Coding

Part:BBa_K3206004:Design

Designed by: Matthew Rogan   Group: iGEM19_Newcastle   (2019-10-15)


GcvA gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 552
    Illegal BamHI site found at 860
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part was designed so it was compatible with Type IIs assembly (no BsaI or SapI restriction). Codon optimisation at site 82 was completed to remove Sap1 binding site in order to make the part Type IIs compatible.

Source

Escherichia coli k12, gene information and sequence retrieved from UniProt- https://www.uniprot.org/uniprot/P0A9F6

References

Wilson, R L & Stauffer, G V, 1994. DNA sequence and characterization of GcvA, a LysR family regulatory protein for the Escherichia coli glycine cleavage enzyme system. The Journal of Bacteriology, 176(10), pp.2862–2868.